Plant Transcription Factor Database
Previous version: v3.0
Oryza glaberrima
HSF Family
Species TF ID Description
ORGLA01G0166200.1HSF family protein
ORGLA01G0188200.1HSF family protein
ORGLA01G0245200.1HSF family protein
ORGLA01G0253200.1HSF family protein
ORGLA02G0087700.1HSF family protein
ORGLA02G0162000.1HSF family protein
ORGLA03G0044000.1HSF family protein
ORGLA03G0087100.1HSF family protein
ORGLA03G0174600.1HSF family protein
ORGLA03G0308600.1HSF family protein
ORGLA03G0383200.1HSF family protein
ORGLA04G0190900.1HSF family protein
ORGLA06G0155700.1HSF family protein
ORGLA07G0042500.1HSF family protein
ORGLA07G0185000.1HSF family protein
ORGLA08G0155600.1HSF family protein
ORGLA08G0219000.1HSF family protein
ORGLA09G0096400.1HSF family protein
ORGLA09G0097500.1HSF family protein
ORGLA10G0088100.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645